research-article | 29-March-2019
their interaction with the insect-pest.
Hence, in an effort to increase the survival time and infectivity of EPNs on ticks, this study aimed to evaluate: (i) the effect of five vegetable oil emulsions on the survival and infectivity of S. carpocapsae, S. websteri and H. bacteriophora on ticks in laboratory and (ii) the control effectiveness (CE) of application of EPNs in oil emulsion on ticks present in infested dogs (Canis lupus familiaris) in field conditions.
Materials and methods
Description
Teodulfo Aquino-Bolaños,
Jaime Ruiz-Vega,
Yolanda D. Ortiz Hernández,
Julio C. Jiménez Castañeda
Journal of Nematology, Volume 51 , 1–10
Research Article | 31-May-2018
Steinernema carpocapsae is an entomopathogenic nematode widely studied for its properties as a biocontrol agent in insect pest management and as a model for understanding bacterial symbioses. Less attention has been given to the development of specific anatomical structures within S. carpocapsae. A better understanding of entomopathogenic nematode development and anatomy may lead to improved biocontrol efficacy. We recently demonstrated that the neuroanatomy of S. carpocapsae IJs differs from
Hung Xuan Bui,
Nathan E. Schroeder
Journal of Nematology, Volume 50 , ISSUE 1, 27–32
Article | 21-July-2017
. carpocapsae, and Heterorhabditis bacteriophora were selected and used in a series of in vitro and in vivo experiments. In preliminary assays, the EPNs species were used with different concentrations of infective juveniles (IJs) (0, 1, 5, 10, 25, and 50 IJ/larvae) in 2-cm diam. plates. The mortality rates of the laboratory tests were 79.75% and 76.5% for S.feltiae and S. carpocapsae, corresponded to LC50 value of 2.02 IJ/larva for S. feltiae and 2.05 IJ/larva for S. carpocapsae. On the contrary
ZAHRA MEMARI,
JAVAD KARIMI,
SHOKOOFEH KAMALI,
SEYED HOSSEIN GOLDANSAZ,
MOJTABA HOSSEINI
Journal of Nematology, Volume 48 , ISSUE 4, 261–267
Article | 21-July-2017
established infestations would limit damage to the tree and prevent the next generation of S. exitiosa from emerging within the orchard. However, such curative measures for control of S. exitiosa do not exist. Our objective was to measure the efficacy of the entomopathogenic nematode, Steinernema carpocapsae, as a curative control for existing infestations of S. exitiosa. In peach orchards, spring applications of S. carpocapsae (obtained from a commercial source) were made to infested trees and compared
DAVID I. SHAPIRO-ILAN,
TED E. COTTRELL,
RUSSELL F. MIZELL,
DAN L. HORTON
Journal of Nematology, Volume 48 , ISSUE 3, 170–176
research-article | 30-November-2020
selected based on previous literature and current market availability. For this study, commercially purchased Steinernema feltiae, Steinernema kraussei, and Heterorhabditis bacteriophora, Steinernema riobrave, Steinernema carpocapsae, and Heterorhabditis indica third stage infective juveniles (IJ3) were tested (Arbico organics, Oro Valley, AZ). We also tested S. riobrave, S. S. carpocapsae, and H. indica IJ3 reared by the Dr. Shapiro-Ilan, USDA, in Byron, Georgia. EPNs were kept in a standard
WinDi Sanchez,
David Shapiro,
Geoff Williams,
Kathy Lawrence
Journal of Nematology, Volume 53 , 1–14
research-article | 30-November-2020
(HOM1 strain); H. georgiana Nguyen, Shapiro-Ilan, and Mbata (Kesha strain); H. floridensis Nguyen, Gozel, Koppenhöfer, and Adams (K22 strain); S. feltiae (SN strain); S. carpocapsae Weiser (All strain); S. riobrave Cabanillas, Poinar, and Raulston (355 strain); S. glaseri Steiner (VS strain); and S. rarum (17C&E strain). All the nematodes were obtained from the USDA-ARS Southeastern Fruit and Tree Nut Research Laboratory culture collection in Byron, GA.
Experiment design and procedures
In the
Yinping Li,
George N. Mbata,
David I. Shapiro-Ilan
Journal of Nematology, Volume 53 , 1–8
research-article | 30-November-2019
).
Susceptibility of Epitrix cucumeris and Pandeleteius cinereus adults to EPNs
The susceptibility of E. cucumeris and P. cinereus adults (Hass avocado [P. americana] pests) to the EPNs isolated, and S. carpocapsae FA2015 as reference (commercially obtained), was evaluated and compared in vitro. Adults of both insect species were collected from a P. americana crop. In vitro bioassays were performed in multicell culture plates containing sterile Whatman No. 1 filter paper (GE Healthcare, United States). In total
Esteban Neira-Monsalve,
Natalia Carolina Wilches-Ramírez,
Wilson Terán,
María del Pilar Márquez,
Ana Teresa Mosquera-Espinosa,
Adriana Sáenz-Aponte
Journal of Nematology, Volume 52 , 1–16
research-article | 30-November-2020
16 hr of light -L- and 8 hr of darkness -D-) in isolated mesh-cages to avoid escapes.
A total of 10 EPN populations from three species (S. feltiae, S. carpocapsae, and H. bacteriophora) were investigated, comprising seven native and three commercial ones (Table 1). All nematodes were reproduced in the insect host Galleria mellonella (Lepidoptera: Pyralidae) (Woodring and Kaya, 1988). The infective juveniles (IJs) were collected in containers with mineral water and stored at 14°C. In any case, the
Raquel Campos-Herrera,
Ignacio Vicente-Díez,
Magda Galeano,
Maryam Chelkha,
María del Mar González-Trujillo,
Miguel Puelles,
David Labarga,
Alicia Pou,
Javier Calvo,
José Eduardo Belda
Journal of Nematology, Volume 53 , 1–14
research-article | 30-November-2020
EPN species like H. bacteriophora and S. carpocapsae (Browning et al., 2004; Glazer et al., 1997; Hara and Kaya, 1982; Rovesti et al., 1988, Rovesti and Deseo, 1990). These studies mostly aimed to assess the compatibility of these compounds with EPNs based on the survival and infectivity of IJs. There, however, is a need to expand on the diversity of nematicides that are tested for impact on EPN, and their effects on the penetration efficacy and reproductive capacity; chemotaxis and longer-term
Mustapha Touray,
Harun Cimen,
Sebnem H. Gulsen,
Derya Ulug,
Dolunay Erdogus,
David Shapiro-Ilan,
Selcuk Hazir
Journal of Nematology, Volume 53 , 1–17
research-article | 30-November-2019
cups and placing them in the incubator, we observed the cups daily for adult emergence until 30 days post treatment application. Adults that successfully emerged were considered to have survived the nematode treatment. All other experimental parameters were the same as described above except we used 10 pupae per replicate (based on the availability of insects).
Pot bioassay
Five nematode species, S. riobrave, S. carpocapsae, S. feltiae, H. indica, and H. bacteriophora were selected for the pot
Muhammad Usman,
Sehrish Gulzar,
Waqas Wakil,
Jaime C. Piñero,
Tracy C. Leskey,
Laura J. Nixon,
Camila Oliveira-Hofman,
Shaohui Wu,
David Shapiro-Ilan
Journal of Nematology, Volume 52 , 1–9
research-article | 30-November-2020
soils, as the ability of the nematodes to find hosts can be inhibited in such soils (Fischer and Führer, 1990), while others tend to thrive in moderate to neutral pH conditions (Hussaini et al., 2004). Clearly, pH can hinder the efficacy of these nematodes, thereby affecting the intended level of biocontrol. Although pH has been shown to affect the survival of Steinernema carpocapsae and Heterorhabditis indica (Hussaini et al., 2004) and S. carpocapsae and S. glaseri (Kung et al., 1990b) differently
Zanele Khathwayo,
Tshimangadzo Ramakuwela,
Justin Hatting,
David I. Shapiro-Ilan,
Nicolene Cochrane
Journal of Nematology, Volume 53 , 1–12