Research Article | 03-December-2018
(Galleria mellonella, Tenebrio molitor, and Lucillia sericata) at three dose levels (1, 3, and 5%). The impact of insect powder was assessed on infective juvenile (IJ) yield in solid media. Additionally, IJs produced in solid culture were subsequently assessed for virulence, and progeny production in a target insect, Spodoptera litura. The dose level of larval powder had a significant effect on IJ yield in both trials, whereas insect type had significant effect on IJ yield in trial 1 but not in trial 2
Shiyu Zhen,
Yang Li,
Yanli Hou,
Xinghui Gu,
Limeng Zhang,
Weibin Ruan,
David Shapiro-Ilan
Journal of Nematology, Volume 50 , ISSUE 4, 495–506
Research Article | 03-December-2018
22°C (333,014 IJs/g) and 25°C (354,165 IJs/g). An average of 26% of the IJs infected G. mellonella larvae at depths of 15 cm within 24 hr. Steinernema innovationi IJs raised greater than 95% of their body off the substrate and moved in a circular pattern, but did not jump. The hosts, Acheta domesticus, Chilo partellus, and Plutella xylostella showed the least susceptibility. All other hosts, [Eldana saccharina, Sesamia calamistis, Tenebrio molitor, G. mellonella, Cydia pomonella] suffered 100
Tshimangadzo Ramakuwela,
Justin Hatting,
Mark D. Laing,
Nicolene Thiebaut,
Selcuk Hazir
Journal of Nematology, Volume 50 , ISSUE 4, 1–10
Research Article | 31-May-2018
. Nonetheless, this nematode is capable of infecting and killing the sparganothis fruitworm Sparganothis sulfureana Clemens (Lepidoptera: Tortricidae), the brown-banded cockroach Supella longipalpa Fabricius (Blattodea: Ectobiidae), and the cranberry fruitworm Acrobasis vaccinii Riley (Lepidoptera: Pyralidae), under laboratory conditions, and each in less than 72 hr. The mealworm Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) and the greater wax moth Galleria mellonella Linnaeus (Lepidoptera
Weimin Ye,
Shane Foye,
Ann E. MacGuidwin,
Shawn Steffan
Journal of Nematology, Volume 50 , ISSUE 1, 9–26
research-article | 30-November-2020
were repeated at least twice using different batches of nematodes and insects.
Origin of insects
Galleria mellonella were fed on an artificial diet (Vanhaeche and Degheele, 1980) and reared in an environmentally controlled room at 28°C and 60% relative humidity (RH). The yellow mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), were purchased from the local market. The 9th to 11th instar larvae were reared at room temperature prior to being used for nematode in vivo culture. The 5th
Xun Yan,
Guimei Chen,
Yuqing Chen,
Bingjiao Sun,
Xinghui Gu,
Weibing Ruan,
Richou Han
Journal of Nematology, Volume 53 , 1–12