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original-report | 25-June-2021

Effect of cryopreservation on a rare McLeod donor red blood cell concentrate

Cryopreservation of red blood cell (RBC) concentrates is used for long-term storage of rare phenotyped RBCs to provide life-saving therapies to patients. To produce these components in Canada, blood manufacturers must demonstrate that the production method they use yields RBC units that meet regulatory guidelines for hemolysis, hemoglobin (Hgb) content, and RBC recovery.1 The Canadian Blood Services (CBS) Rare Blood Program maintains a carefully selected inventory of cryopreserved RBCs with

T.R. Turner, G. Clarke, G.A. Denomme, R. Skeate, J.P. Acker

Immunohematology, Volume 37 , ISSUE 2, 78–83

Report | 24-March-2020

Cryopreserving and deglycerolizing sickle cell trait red blood cell components using an automated cell-processing system

; CP2D/AS-3 RBC components were collected from three donors with sickle cell trait.  Each component was processed with an automated cell-processing system (ACP 215, Haemonetics Corp., Braintree, MA) and cryopreserved within 6 days of collection.  The components were stored at –65°C or less for at least 2 days and were deglycerolized using the automated cell-processing system’s standard procedure.  Before cryopreservation and after deglycerolization, several variables

Ricci Jo Ackley, A. Hallie Lee-Stroka, Barbara J. Bryant, David F. Stroncek, Karen M. Byrne

Immunohematology, Volume 24 , ISSUE 3, 107–111

Report | 01-December-2019

Cryopreservation of red blood cell units with a modified method of glycerolization and deglycerolization with the ACP 215 device complies with American and European requirements

Jana List, Michaela Horvath, Gerda C. Leitner, Günter Weigel

Immunohematology, Volume 28 , ISSUE 2, 67–73

Report | 16-March-2020

Transfusion of rare cryopreserved red blood cell units stored at -80°C: the French experience

through the French hemovigilance program, which requires a mandatory report of any transfusion reaction. It has recently been suggested to extend the 10-year restriction in some countries. Considering our experience and observational data, we may consider it safe and efficient to transfuse rare frozen RBC units older than 10 years. An international consensus for RBC cryopreservation time should ideally be established.

Thierry Peyrard, Bach-Nga Pham, Pierre-Yves Le Pennec, Philippe Rouger

Immunohematology, Volume 25 , ISSUE 1, 13–17

Research Article | 26-September-2018

A Model for Evolutionary Ecology of Disease: The Case for Caenorhabditis Nematodes and Their Natural Parasites

, cryopreservation, behavioral assays, and genomic tools. Throughout, we present avenues of research that are interesting and feasible to address with caenorhabditid nematodes and their natural parasites, ranging from the maintenance of outcrossing to the community dynamics of host-associated microbes. In combining natural relevance with the experimental power of a laboratory supermodel, these fledgling host–parasite systems can take on fundamental questions in evolutionary ecology of disease.

AMANDA K. GIBSON, LEVI T. MORRAN

Journal of Nematology, Volume 49 , ISSUE 4, 357–372

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