original-report | 25-June-2021
future autologous and allogeneic use.3–5,7,8 However, component quality post-deglycerolization for this phenotype has not been previously described.
In addition to the challenge of understanding how RBCs having rare blood phenotypes can tolerate the cryopreservation process, blood manufacturers are also faced with the implications of long-term storage of these components. Cryopreserved RBCs can be stored for up to 10 years (30 years in Canada and other jurisdictions with medical approval), ensuring
Immunohematology, Volume 37 , ISSUE 2, 78–83
Report | 24-March-2020
RBC components with rare phenotypes are sometimes required for patients with sickle cell disease, and these rare components can often be found among donors with sickle cell trait. Cryopreserving RBC components from sickle cell trait donors requires a modified deglycerolization method to preserve the integrity of the RBCs. This study evaluated the feasibility of using an automated cell-processing system to cryopreserve and deglycerolize sickle cell trait donor RBC components.
Ricci Jo Ackley,
A. Hallie Lee-Stroka,
Barbara J. Bryant,
David F. Stroncek,
Karen M. Byrne
Immunohematology, Volume 24 , ISSUE 3, 107–111
Report | 01-December-2019
Current red blood cell (RBC) glycerolization with the ACP 215 device is followed by volume reduction of the glycerolized RBCs before freezing. We investigated a modified method of glycerolization and deglycerolization which eliminates the final centrifugation step that reduces glycerolized RBC supernatant. A total of 37 RBC units collected from healthy volunteers were analyzed. After removal of the supernatant, RBCs were glycerolized using the high glycerol method and stored at –80°C
Gerda C. Leitner,
Immunohematology, Volume 28 , ISSUE 2, 67–73
Article | 22-January-2021
% NaCl solution.
9% NaCl solution
2.5% NaCl solution
Blood bank saline*
12% NaCl solution
1.6% NaCl solution
Blood bank saline*
*0.9% NaCl solution.
Procedural Steps: Glycerolization
Wash and pack the RBCs to be glycerolized.
Add 2 volumes of glycerol solution to 1 volume of RBCs in a dropwise manner.
Freeze at ≤ –70°C
Immunohematology, Volume 36 , ISSUE 3, 85–88