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original-report | 25-June-2021

Effect of cryopreservation on a rare McLeod donor red blood cell concentrate

future autologous and allogeneic use.3–5,7,8 However, component quality post-deglycerolization for this phenotype has not been previously described. In addition to the challenge of understanding how RBCs having rare blood phenotypes can tolerate the cryopreservation process, blood manufacturers are also faced with the implications of long-term storage of these components. Cryopreserved RBCs can be stored for up to 10 years (30 years in Canada and other jurisdictions with medical approval), ensuring

T.R. Turner, G. Clarke, G.A. Denomme, R. Skeate, J.P. Acker

Immunohematology, Volume 37 , ISSUE 2, 78–83

Report | 24-March-2020

Cryopreserving and deglycerolizing sickle cell trait red blood cell components using an automated cell-processing system

RBC components with rare phenotypes are sometimes required for patients with sickle cell disease, and these rare components can often be found among donors with sickle cell trait.  Cryopreserving RBC components from sickle cell trait donors requires a modified deglycerolization method to preserve the integrity of the RBCs.  This study evaluated the feasibility of using an automated cell-processing system to cryopreserve and deglycerolize sickle cell trait donor RBC components. 

Ricci Jo Ackley, A. Hallie Lee-Stroka, Barbara J. Bryant, David F. Stroncek, Karen M. Byrne

Immunohematology, Volume 24 , ISSUE 3, 107–111

Report | 01-December-2019

Cryopreservation of red blood cell units with a modified method of glycerolization and deglycerolization with the ACP 215 device complies with American and European requirements

Current red blood cell (RBC) glycerolization with the ACP 215 device is followed by volume reduction of the glycerolized RBCs before freezing. We investigated a modified method of glycerolization and deglycerolization which eliminates the final centrifugation step that reduces glycerolized RBC supernatant. A total of 37 RBC units collected from healthy volunteers were analyzed. After removal of the supernatant, RBCs were glycerolized using the high glycerol method and stored at –80°C

Jana List, Michaela Horvath, Gerda C. Leitner, Günter Weigel

Immunohematology, Volume 28 , ISSUE 2, 67–73

Article | 22-January-2021

Freezing and recovering rare red blood cells using glycerol

% NaCl solution. Reagents/Supplies: Deglycerolization Reagents Supplies Method 1 9% NaCl solution 2.5% NaCl solution Blood bank saline* Test tubes Transfer pipettes Timer Centrifuge Method 2 12% NaCl solution 1.6% NaCl solution Blood bank saline* *0.9% NaCl solution. Procedural Steps: Glycerolization Method 1 Wash and pack the RBCs to be glycerolized. Add 2 volumes of glycerol solution to 1 volume of RBCs in a dropwise manner. Mix gently. Freeze at ≤ –70°C

B. Eades

Immunohematology, Volume 36 , ISSUE 3, 85–88

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