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research-article | 17-April-2019

First Report of Criconema demani from Russia

a 11.5 ± 1.4 (8.9–13.2) 9–13 8.8–10 10.2–11.7 b 3.8 ± 0.4 (3.3–4.6) 3.8–5 3.4–4 3.6–4.4 c 12.8 ± 1.9 (10.3–15.8) 9–16 11–13 12–16.5 VL/VB 1.71 ± 0.2 (1.4–2.0) 1.6–2.2 1.6–1.8 – Figure 1 Light micrographs of Criconema demani. (A) Entire female and (B) Tail region. Molecular characterization. Despite the wide geographical distribution of the studied nematode species, as it is known from the literature, there are no molecular data for this species in the GenBank. There are two

Sergei Tabolin, Irina Markina

Journal of Nematology, Volume 51 , 1–4

research-article | 30-November-2020

Morphological and Molecular Characterization of Quinisulcius curvus from China

population of stunt nematodes was recovered from the rhizosphere of a shrub, sea randa in 2019. No above ground symptoms were observed on the host. The population was detected in high density (200-300/100 g of soil) as compared to other soil nematodes. Preliminary examination showed that the species has five lateral lines which is a salient characteristic of genus Quinisulcius. Therefore morphological and molecular characterization were performed, and the results were compared with the nominal species of

Jianfeng Gu, Maria Munawar, Pablo Castillo, Bo Cai

Journal of Nematology, Volume 53 , 1–11

Article | 03-December-2017

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

RAJAN SALALIA, R. K. WALIA, VISHAL SINGH SOMVANSHI, PUNEET KUMAR, ANIL KUMAR

Journal of Nematology, Volume 49 , ISSUE 3, 254–267

Research Article | 03-September-2018

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization

Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A. Subbotin, Alejandro Tovar-Soto

Journal of Nematology, Volume 50 , ISSUE 2, 229–242

Case report | 01-December-2019

Serologic and molecular characterization of D variants in Brazilians: impact for typing and transfusion strategy

Rh discrepancies are a problem during routine testing because of partial D or weak D phenotypes. Panels of monoclonal antibodies (MoAb) are being developed to identify D variants such as partial D and weak D when there are anomalous D typing results; however, molecular characterization offers a more specific classification of weak and partial D. The weak D and partial D phenotypes are caused by many different RHD alleles encoding aberrant D proteins, resulting in distinct serologic phenotypes

Débora Castilho Credidio, Jordão Pellegrino Jr., Lilian Castilho

Immunohematology, Volume 27 , ISSUE 1, 6–11

research-article | 30-November-2018

First report of Mesocriconema sphaerocephalum (Taylor, 1936) Loof, 1989 associated with carrot (Daucus carota subsp. Stativus) in Vietnam

) Rv 4.8 ± 0.4 (4–5) 4.3 ± 0.4 (4–5) Ran 2.2 ± 0.4 (2–3) 2 ± 0.3 (2–3) Rvan 3 ± 0.3 (2–3) 2.2 ± 0.4 (2–3) R 65 ± 1.6 (63–68) 64 ± 1.4 (62–66) Figure 1: Mesocriconema sphaerocephalum on carrot in Vietnam. (A) entire body; (B) head region; (C) stylet; (D) anastomoses at mid-body; (E) pharyngeal region; (F) tail region; (G) anastomoses at tail region (Scale bar: 10 µm). Molecular characterization D2D3 of 28S rDNA sequence of M. sphaerocephalum in this study was 730 bp, submitted to

Thi Duyen Nguyen, Huu Tien Nguyen, Thi Mai Linh Le, Thi Tuyet Thu Tran, Neriza Nobleza, Quang Phap Trinh

Journal of Nematology, Volume 51 , 1–4

original-paper | 28-March-2019

Predominance of Lactobacillus plantarum Strains in Peruvian Amazonian Fruits

(Beltec Scientific). These cultures were also used to perform Gram staining and catalase activity with 3% hydrogen peroxide. Acid production was performed by adding 2% CaCO3 to MRS plates. Gram-positive, catalase-negative and acid producer isolates were considered presumptive LAB. Molecular characterization and identification. From an overnight culture, 1 ml of each culture was used for bacterial DNA extraction according to the procedure of Ausubel et al. (2003). DNA was resuspended in 50 µl of TE and

JOHANNA SÁNCHEZ, CARLOS VEGAS, AMPARO IRIS ZAVALETA, BRAULIO ESTEVE-ZARZOSO

Polish Journal of Microbiology, Volume 68 , ISSUE 1, 127–137

research-article | 30-November-2019

First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam

. A1 light microscope equipped with a Zeiss Axiocam ERc5s digital camera. For molecular characterization, the 5′-end region of 28S rDNA was amplified using DP391/501 primers (5′-AGCGGAGGAAAAGAAACTAA-3′/5′-TCGGAAGGAACCAGCTACTA-3′) following Nguyen et al. (2019b). Forward and reverse sequences were assembled and analyzed using Geneious R11 (Nguyen et al., 2019b, 2019c). The best fit model was chosen using Mega 7 following Nguyen et al. (2019b). Results and discussion Measurements Eight females: L

Huu Tien Nguyen, Thi Duyen Nguyen, Thi Mai Linh Le, Quang Phap Trinh

Journal of Nematology, Volume 52 , 1–4

research-article | 30-November-2020

First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne javanica, infecting ginger and long coriander in Vietnam

nematodes. In this study, morphology and morphometric of second-stage juveniles, males, and females of Meloidogyne javanica on ginger and long coriander in Vietnam were provided for the first time. The study revealed new distribution and new host for M. javanica. Besides, molecular characterization of D2-D3 fragment of rDNA, ITS rDNA, and Nad5 mtDNA gene regions were given to support the morphological identification of M. javanica populations in this study. Material and methods Soil and root samples

Ke Long Phan, Thi Mai Linh LE, Huu Tien Nguyen, Thi Duyen Nguyen, Quang Phap Trinh

Journal of Nematology, Volume 53 , 1–8

Original Paper | 15-March-2016

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Rama Sowmya, Nakkarike M. Sachindra

Polish Journal of Microbiology, Volume 65 , ISSUE 1, 77–88

research-article | 11-January-2021

On the molecular identity of Paratylenchus nanus Cobb, 1923 (Nematoda: Tylenchida)

Church, Virginia. Scale – 10 µm. Figure 2: Posterior regions of Paratylenchus. A: P. projectus from samples collected in Devils Lake, Ramsey County, North Dakota; B-F: P. nanus from samples collected in Four Mile Run, Fall Church, Virginia. Scale – 10 µm. Molecular characterization and relationships The D2-D3 of 28 S rRNA gene The alignment generated with modified parameters was 756 bp in length and contained 41 sequences. Phylogenetic relationships of P. nanus within selected

Sergei A. Subbotin, Guiping Yan, Mihail Kantor, Zafar Handoo

Journal of Nematology, Volume 52 , 1–7

Article | 21-July-2017

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

LYNN K. CARTA, SHIGUANG LI, ANDREA M. SKANTAR, GEORGE NEWCOMBE

Journal of Nematology, Volume 48 , ISSUE 1, 28–33

Original Paper | 26-August-2016

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

Aleksandra Januszkiewicz, Waldemar Rastawicki

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 261–269

research-article | 30-November-2019

Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey

world reported on D2 to D3 expansion regions of 28S rDNA (Al-Banna et al., 1997; Subbotin et al., 2008; Lopez-Nicora et al., 2012; Chihani-Hammas et al., 2018) confirmed the specificity of the molecular identifications. Molecular identification is specific and reliable in distinguishing nematode species (Nguyen et al., 2017). Nematode species difficult to distinguish morphologically from other species can be successfully identified using molecular characterization. Detection of P. vulnus on wheat

Mehmet Sait Karaca, Elif Yavuzaslanoglu, Gul Imriz, Ozlem Ates Sonmezoglu

Journal of Nematology, Volume 52 , 1–4

research-article | 30-November-2020

Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

euphrates poplar worldwide. Molecular characterization and phylogenetic relationships Two 713 nt long D2-D3 expansion segments of LSU rDNA (MZ725030, MZ725031) were obtained for the Iranian population of this species. The BLAST search using these sequences revealed they have 99.85% identity with other sequences of the same species (MG770479, KJ461545 and KJ461547). A total of 74 sequences of Tylenchoidea Örley, 1880 and two sequences of Aphelenchoidea (Fuchs, 1937) Thorne, 1949 as outgroup taxa

Abbas Abdolkhani, Sedighe Azimi

Journal of Nematology, Volume 53 , 1–10

Original Paper | 04-September-2018

Biochemical and Molecular Characterization of a Native Haloalkalophilic Tolerant Strain from the Texcoco Lake

MARISELA YADIRA SOTO-PADILLA, PABLO GORTÁRES-MOROYOQUI, LUIS ALBERTO CIRA-CHÁVEZ, MARÍA ISABEL ESTRADA-ALVARADO

Polish Journal of Microbiology, Volume 67 , ISSUE 3, 377–382

Article | 01-April-2020

Serologic and molecular characterization of the B(A) blood group in the Chinese population

Zhong-Hui Guo, Dong Xiang, Zi-Yan Zhu, Xi Liu, He-Ping Chen, Jian-Lian Wang, Da-Zhuang Liu, Tong-Mao Zhao

Immunohematology, Volume 23 , ISSUE 2, 69–74

Article | 15-April-2020

Molecular characterization of GYPB and RH in donors in the American Rare Donor Program

Sunitha Vege, Connie M. Westhoff

Immunohematology, Volume 22 , ISSUE 3, 143–147

research-article | 16-April-2019

First report of cultivated Cretan mountain tea (Sideritis syriaca) as a host of Meloidogyne hapla and M. javanica in Crete, with some additional records on the occurrence of Meloidogyne species in Greece

infection (8–30 galls per gram of root) while in the surrounding soil were found juveniles of Meloidogyne at a density of 1.6 per gram of soil. Meloidogyne hapla was detected in the sample examined in March 2017. The species had been previously reported in Greece (Hirschmann et al., 1966; Koliopanos, 1980; Vovlas and Antoniou, 1987; Vlachopoulos, 1994) and Crete (Pyrowolakis, 1980) with identification based only on morphological characteristics. Herein, this study is the first report of molecular

Emmanuel A. Tzortzakakis, Carolina Cantalapiedra-Navarrete, Antonio Archidona-Yuste, Maria Kormpi, Juan E. Palomares-Rius, Pablo Castillo

Journal of Nematology, Volume 51 , 1–4

Research Article | 17-October-2018

Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

Yousef Panahandeh, Joaquín Abolafia, Ebrahim Pourjam, Robin M. Giblin-Davis, Farahnaz Jahanshahi Afshar, Majid Pedram

Journal of Nematology, Volume 50 , ISSUE 3, 343–354

Original Paper | 28-December-2016

Molecular Characterization of Enteroviruses Isolated from Acute Flaccid Paralysis Cases in Poland, 1999–2014

Magdalena Wieczorek, Arleta Krzysztoszek

Polish Journal of Microbiology, Volume 65 , ISSUE 4, 443–450

research-article | 03-June-2019

Morphological and molecular characterization of Labronema montanum sp. n. (Dorylaimida, Dorylaimidae) from Spain

is 4.5 to 6 µm broad; curvature is 130 to 140°; ventral hump and hollow are poorly demarcated but appreciable; the former is situated at 36 to 58% of the spicule length from its anterior end. Lateral guiding piece is curved ventrad, 19 to 25 µm long, 5.4 to 6.3 times longer than broad, and with somewhat bifid terminus. Molecular characterization Three D2-D3 28S-rRNA sequences were obtained, one with 769, 772 and 783 pb long. These three fragments agree in 729 pb long, all of them were 100

R. Peña-Santiago, J. Abolafia

Journal of Nematology, Volume 51 , 1–11

research-article | 06-November-2020

Morphological and molecular characterization of Epidorylaimus procerus sp. n. (Dorylaimida: Qudsianematidae) from Vietnam

Thi Anh Duong Nguyen, Reyes Peña-Santiago

Journal of Nematology, Volume 52 , 1–8

research-article | 16-January-2021

Occurrence and molecular characterization of Meloidogyne graminicola on rice in Central Punjab, Pakistan

. graminicola by light (above) and electron (below) microscopy. Molecular characterization through ITS amplification and sequencing PCR amplification and sequencing yielded eight unique sequences. The isolates gave similar-sized bands of 326–328 bp on a gel stained with ethidium bromide (Figure 4). GenBank accession numbers for each of the obtained sequences are given in Tables 3–6. M. graminicola isolates JB1CT, JB2 UAF, JB3FSD1, JB3FSD2, JB3FSD3, JB3FSD4, JB3FSD5, and JB3FSD6 (accessions KX757064

Abdul Jabbar, Nazir Javed, Anjum Munir, Huma Abbas, Sajid A. Khan, Anam Moosa, Muhammad Jabran, Byron J. Adams, Muhammad A. Ali

Journal of Nematology, Volume 52 , 1–17

research-article | 30-November-2020

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

A. Hajjaji, R. Ait Mhand, N. Rhallabi, F. Mellouki

Journal of Nematology, Volume 53 , 1–8

research-article | 24-April-2020

First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)

Malorri R. Hughes, Deborah A. Duffield, Dana K. Howe, Dee R. Denver

Journal of Nematology, Volume 52 , 1–6

Research Article | 31-May-2018

Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China

opening large, and relative proximity of vulva to the anus. Detailed morphological and molecular characterization of the new genus/species is presented along with the comparison of the related genera.

Qing Yu, Maria Munawar, Jianfeng Gu, Weimin Ye

Journal of Nematology, Volume 50 , ISSUE 1, 69–76

Research Article | 03-December-2018

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs

Lynn K. Carta, R. L. Wick

Journal of Nematology, Volume 50 , ISSUE 4, 473–478

research-article | 30-November-2020

Report of the Texas peanut root-knot nematode, Meloidogyne haplanaria (Tylenchida: Meloidogynidae) from American pitcher plants (Sarracenia sp.) in California

, bermudagrass, and birch in Arkansas (Khanal et al., 2016; Ye et al., 2019). Meloidogyne haplanaria has been shown to overcome the Mi resistant gene to M. arenaria, M. javanica, and M. incognita in tomato. However, the peanut cultivar ‘NemaTAM’, which is resistant to M. arenaria and M.javanivca, is also resistant to the Texas root-knot nematode (Bendezu et al., 2004). The objective of the present study was to provide molecular characterization of M. haplanaria parasitising potted American pitcher plants in

Sergei A. Subbotin

Journal of Nematology, Volume 53 , 1–7

research-article | 30-November-2020

Phylogenetics and genetic variation of Heligmosomoides thomomyos in Western pocket gophers (Thomomys spp.)

Malorri R. Hughes, Alexandra A. Gibson, Emily R. Wolfe, Cecily D. Bronson, Deborah A. Duffield

Journal of Nematology, Volume 53 , 1–11

Short Communication | 28-June-2017

Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

Beata Zimowska, Ewa Dorota Zalewska, Ewa Dorota Król, Agnieszka Furmańczyk

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 281–285

Research Article | 26-September-2018

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus sucus and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of S. sucus closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian S. sucus using the 18S

ELENA FANELLI, ALBERTO TROCCOLI, NICOLA VOVLAS, GIANLUCA SCARCIA, ANNAMARIA MINCUZZI, SIMONA M. SANZANI, ANTONIO IPPOLITO, FRANCESCA DE LUCA

Journal of Nematology, Volume 49 , ISSUE 4, 418–426

research-article | 30-November-2018

Morpho-molecular characterization of Colombian and Brazilian populations of Rotylenchulus associated with Musa spp

Donald Riascos-Ortiz, Ana Teresa Mosquera-Espinosa, Francia Varón De Agudelo, Claudio Marcelo Gonçalves de Oliveira, Jaime Eduardo Muñoz-Flórez

Journal of Nematology, Volume 51 , 1–13

research-article | 13-April-2020

First molecular characterization of an Iranian population of Schistonchus caprifici (Gasparrini, 1864) Cobb, 1927 (Rhabditida; Aphelenchoidea)

Hadi Karimipour Fard, Hamid Zare

Journal of Nematology, Volume 52 , 1–3

research-article | 30-November-2019

Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands

clearly different from all known species, see below. Molecular characterization D2-D3 of 28S rDNA Three D2-D3 sequences (MT508987-MT508989) of 987-1039 bp were produced without intraspecific sequence variation. The closest available sequence on GenBank was MK292129 of H. glycines with 95.9% similarity (43 out of 1039 bp differences). The D2-D3 alignment of 750 bp long consisted of 75 Heterodera sequences of 33 species and a Cryphodera sinensis sequence (JX566455) as the outgroup. The resulting D2

Phougeishangbam Rolish Singh, Gerrit Karssen, Marjolein Couvreur, Wim Bert

Journal of Nematology, Volume 52 , 1–14

research-article | 30-November-2019

Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa

, before DNA extraction (see Table 1). Molecular characterization Following morphological identification, the same individual nematodes were picked from temporary slides and used for extraction of genomic DNA using a quick alkaline lysis protocol (Janssen et al., 2016). DNA was amplified by preparing 24 μl PCR master mix comprising 16 μl double sterilized distilled water, 2.5 μl 10x buffers, 2 μl MgCl2, 0.05 μl of dNTP (10 mM), 1 μl of reverse and forward primers, 0.05 μl of Toptaq and 2 μl of

Yao A. Kolombia, Oluwadamilola Ogundero, Emmanuel Olajide, Nicole Viaene, P. Lava Kumar, Danny L. Coyne, Wim Bert

Journal of Nematology, Volume 52 , 1–25

research-article | 26-March-2021

Morphological and molecular characterization of Butlerius butleri Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report

from vermicompost in Iran and presented the first molecular characterization of the species. In South Africa the genus Butlerius was first reported in 1961 (Heyns, 1961) and are commonly found in soil samples (Dr Marais, ARC-PHP, personal communication). However, populations of the genus are very rarely formally reported in the country. Species of the genus are yet to be reported or described from South Africa. During sampling of compost matter from two different localities in Potchefstroom, North

Chantelle Girgan, Gerhard Du Preez, Hendrika Fourie, Milad Rashidifard

Journal of Nematology, Volume 53 , 1–12

research-article | 30-November-2020

Morphological and molecular characterization of Iotonchus lotilabiatus n. sp. (Nematoda: Iotonchidae) from Lao Cai Province, Vietnam

Mononchida, from the analysis of the 28S rDNA sequences under ML (K2+G model (BIC = 2,373.292, AICc = 2,087.600; lnL = ‒1,000.469, G = 0.23, R = 2.00, f(A) = 0.25, f(T) = 0.25, f(C) = 0.25, f(G) = 0.25)). Numbers to the left of the branches are bootstrap values for 1,000 replications. Conclusions During this study, the new species Iotonchus lotilabiatus n. sp. is supported by its morphological and molecular characterization. An updated key to species of the genus Iotonchus (Cobb, 1916) based on

Tam T. T. Vu, Thi Mai Linh Le, Thi Duyen Nguyen

Journal of Nematology, Volume 53 , 1–22

Article | 21-July-2017

First Report and Comparative Study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its Symbiont Bacteria from Subcontinental India

isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of ITS-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense COI gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont

AASHIQ HUSSAIN BHAT, I STKHAR, ASHOK KUMAR CHAUBEY, VLADIMIR PUZA, ERNESTO SAN-BLAS

Journal of Nematology, Volume 49 , ISSUE 1, 92–102

Research Article | 03-September-2018

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to A. tetraptera recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.

Rewaida Abdel-Gaber, Fathy Abdel-Ghaffar, Saleh Al Quraishy, Kareem Morsy, Rehab Saleh, Heinz Mehlhorn

Journal of Nematology, Volume 50 , ISSUE 2, 117–132

Research Article | 20-May-2019

FRANCISELLA TULARENSIS – REVIEW 

variable intergenic regions may be also applicable to detection, differentiation and determination of genetic variation among F. tularensis strains. In addition, the above methods could be successfully used in molecular characterization of tularaemia strains from humans and animals isolated in screening research, and during epizootic and epidemic outbreaks.

Piotr Cieślik, Józef Knap, Agata Bielawska-Drózd

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 1, 58–67

Article | 21-July-2017

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

QING YU, WEIMIN YE, TOM POWERS

Journal of Nematology, Volume 48 , ISSUE 3, 203–213

Research Article | 03-September-2018

Molecular Characterization and Phylogeny of Ditylenchus weischeri from Cirsium arvense in the Prairie Provinces of Canada

Mehrdad Madani, Mario Tenuta

Journal of Nematology, Volume 50 , ISSUE 2, 163–182

Research Article | 26-September-2018

First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

SOLOMIA SUSULOVSKA, PABLO CASTILLO, ANTONIO ARCHIDONA-YUSTE

Journal of Nematology, Volume 49 , ISSUE 4, 396–402

research-article | 30-November-2018

Integrative taxonomy of Xiphinema histriae and Xiphinema lapidosum from Spain

record of the occurrence of X. histriae and X. lapidosum in Spain and the first time that describes the molecular characterization and the juvenile stages of both species. Xiphinema histriae was originally described from Italy associated with grapevine (Lamberti et al., 1993a, 1993b, and later on, reported from the rhizosphere of wild growing grape (Vitis vinifera ssp. silvestris) in Austria (Tiefenbrunner and Tiefenbrunner, 2004). Based on the detailed study of paratypes and both Spanish

Ruihang Cai, Antonio Archidona-Yuste, Carolina Cantalapiedra-Navarrete, Juan E. Palomares-Rius, Jingwu Zheng, Pablo Castillo

Journal of Nematology, Volume 51 , 1–21

Original Paper | 09-March-2018

Molecular Characterization of the cry Gene profile of Bacillus thuringiensis Isolated from a Caribbean Region of Colombia

Pedro Fragoso, Alicia Armijo, Doris Gómez, Claudio Gómez, Marco Bugueño, Gittith Sánchez, Juan Venegas

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 19–26

research-article | 30-November-2020

Morphological and molecular characterization of Xiphinemella esseri Chitwood, 1957 (Dorylaimida: Leptonchidae) from Florida, with the first molecular study of the genus

Bruin and Heyns (1991). The differences found in the spicule length might be due to the fact that in our study, and also in the original description of the species, the measure of the arc of the spicule are provided, whereas in the De Bruin and Heyns’ description most likely (it is not specifically mentioned in the description) the measure of the chord of the spicule was taken. Molecular characterization and phylogenetic position of Xiphinemella esseri The D2-D3 of 28S rRNA gene alignment

Sergio Álvarez-Ortega, Sergei A. Subbotin, Renato N. Inserra

Journal of Nematology, Volume 53 , 1–9

research-article | 30-November-2018

Morphological and Molecular Characterization of Coslenchus paramaritus n. sp. and C. cancellatus (Cobb, 1925) Siddiqi, 1978 (Nematoda: Tylenchidae) from Iran

Manouchehr Hosseinvand, Ali Eskandari, Reza Ghaderi

journal of nematology, Volume 51 , 1–10

research-article | 28-April-2020

Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status

Demanian indices. Molecular characterization and its taxonomical implications A. saeedi strains DH1 and KMW were molecularly characterized by ITS rDNA (901 bp, 938 bp), 18 S rDNA (894 bp, 895 bp) and flanking regions D2-D3 of rDNA (984 bp, 997 bp), respectively. The NBlast analysis of D2-D3, 18 S and ITS rDNA sequences of present specimens showed 100% similarity with D2-D3 (KY914573), 18 S (KY090631) and ITS (KY090632) rDNA sequences of Acrobeloides sp. ES-2017 isolate SMF3 from Iran. 18 S sequences

Aasha Rana, Aashaq Hussain Bhat, Suman Bhargava, Ashok Kumar Chaubey, Joaquín Abolafia

Journal of Nematology, Volume 52 , 1–21

research-article | 30-November-2019

Morphological and molecular characterization of two species of Neothada Khan, 1973 (Nematoda: Tylenchidae) from Iran, with notes on N. cancellata

Manouchehr Hosseinvand, Ali Eskandari, Reza Ghaderi

Journal of Nematology, Volume 52 , 1–10

research-article | 25-May-2020

Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species

poinari (Aliramaji et al., 2014) revealed that a slightly projecting structure is present slightly anterior to the cloacal opening. This could be the single precloacal P1 papilla, or the paired papillae, similar to those in E. pini. The last pair is situated at 14.5 µm distance to the tail tip. The arrangement and number of papilla for this species are amended herein. Molecular characterization and phylogenetic relationships Partial SSU rDNA phylogeny To determine the phylogenetic relationships of

Fariba Heydari, Majid Pedram

Journal of Nematology, Volume 52 , 1–12

research-article | 30-November-2020

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

; Brzeski, 1995; Doucet, 1994; Esser, 1992; Geraert, 1965; Huang and Raski, 1986; Raski, 1991; Shahina and Maqbool, 1993; Van den Berg and Buckley, 1993) or a subgenus of Paratylenchus (Siddiqi, 2000). In the book on Tylenchulidae, Ghaderi et al. (2016) recognized 117 species of Paratylenchus. With the advent of molecular biology, phylogenetic studies have been conducted to examine the relationships among paratylenchids. Subbotin et al. (2005) were the first to provide molecular characterization of

Mihail R. Kantor, Zafar A. Handoo, Sergei A. Subbotin, Gary R. Bauchan, Joseph D. Mowery

Journal of Nematology, Volume 53 , 1–10

research-article | 30-November-2019

Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

H. pararobustus also grouped in a clade with H. galeatus that is representative of the group of species with four incisures in the lateral field and three esophageal gland nuclei (Van den Berg and Quénéhervé, 2012). Hoplolaimus seinhorsti reported from Africa, Asia, and Central and South America is representative of a group with one or no incisures in the lateral field and with six pharyngeal gland nuclei. Molecular characterization Nucleotide BLAST search using partial 18 S sequence of H

Mariette Marais, Esther van den Berg, Hendrika Fourie, Milad Rashidifard

Journal of Nematology, Volume 52 , 1–12

research-article | 28-April-2020

Morphological and molecular characterization of Pungentus sufiyanensis n. sp. and additional data on P. engadinensis (Altherr, 1950) Altherr, 1952 (Dorylaimida: Nordiidae) from northwest of Iran

. The caudal region short, rounded-conoid, slightly more straight at the ventral side, where it bears saccate bodies; two pairs of caudal pores are present. Male: unknown. Molecular characterization: one sequence of the D2-D3 segment of 28S rDNA nearly 800 bp long from the new species was obtained. The results of its analysis are represented in the molecular tree of Figure 3. Figure 1: Pungentus sufiyanensis n. sp. (A) neck region; (B) anterior region; (C) amphidial pouch, (D) entire body, (E

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Reyes Peña-Santiago

Journal of Nematology, Volume 52 , 1–12

research-article | 30-November-2020

Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae)

Parnaz Mortazavi, Fariba Heydari, Joaquín Abolafia, Pablo Castillo, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

research-article | 30-November-2018

Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

of hermaphroditism in this species. Molecular characterization One 923 bp 18S rDNA sequence (GenBank accession number MK959600) and three identical 637 bp without changes or deletions each other 28S rDNA sequences (GenBank accession numbers MK959601-MK959603) were obtained from three specimens. Both trees show O. dolichura as the sister species of O. saproxylicus sp. n. With respect to O. dolichura, the 18S fragment (with 914 bp), show four changes or deletions while the 28S fragments (with 637

Joaquín Abolafia, Reyes Peña-Santiago

journal of nematology, Volume 51 , 1–21

research-article | 11-January-2018

Morphological and Molecular Characterization of Paralongidorus sali Siddiqi, Hooper, and Khan, 1963 with a Description of the First-Stage Juvenile and Male of Longidorus jonesi Siddiqi, 1962 from China

, Longidoroides, and Paralongidorus is the shape of amphids (pouch like in Longidorus and Longidoroides vs. funnel/stirrup shaped in Paralongidorus) and the opening of amphidial aperture (pore-like in Longidorus vs. slit-like in Longidoroides and Paralongidorus) (Oliveira and Neilson, 2006). Several new species of Paralongidorus have published with complete molecular characterization and scanning electron microscopy (SEM) observations (Palomares-Rius et al., 2008, 2013; Pedram et al., 2012; Gutiérrez

Ruihang Cai, Munawar Maria, Nan Qu, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 50 , ISSUE 3, 419–436

research-article | 30-November-2020

New insights into the identity of Discolaimium dubium Das, Khan and Loof, 1969 (Dorylaimida) as derived from its morphological and molecular characterization, with the proposal of its transference to Aporcella Andrássy, 2002

10% of total spicule length, with its dorsal side slightly longer than the ventral one; ventral side bearing visible hump and hollow, median piece occupying 85 to 87% of spicule length. Lateral guiding pieces 8.5 to 9 µm long. Prerectum 2.4 to 4.5, cloaca 1.4 to 1.5 times body diameter at level of cloacal aperture. Molecular characterization Three D2-D3 28S rDNA sequences were obtained, ca 800 bp long. Their analysis has allowed the elucidation of evolutionary relationships of the species. The

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Arezoo Naghavi, Reyes Peña-Santiago

Journal of Nematology, Volume 53 , 1–12

research-article | 14-December-2020

Morphological, morphometrical, and molecular characterization of Metarhabditis amsactae (Ali, Pervez, Andrabi, Sharma and Verma, 2011) Sudhaus, 2011 (Rhabditida, Rhabditidae) from India and proposal of Metarhabditis longicaudata as a junior synonym of M. amsactae

carbon dioxide, and coated with gold. The mounts were examined with a Zeiss Merlin microscope (5 kV). Nematode molecular characterization Genomic DNA was isolated from approximately five hundred infective juvenile nematodes by using the Qiagen Blood and Tissue Analysis Kit following the manufacturer’s protocol. A fragment of the rDNA gene containing the ITS regions (ITS1, 5.8S, ITS2) was amplified using primers 18S: 5′-TTGATTACGTCCCTGCCCTTT-3′ (forward), and 26S: 5′-TTTCACTCGCCGTTACTAAGG-3

Aashaq Hussain Bhat, Shreyansh Srivastava, Aasha Rana, Ashok Kumar Chaubey, Ricardo A. R. Machado, Joaquín Abolafia

Journal of Nematology, Volume 52 , 1–23

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