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  • Immunohematology


Article | 16-November-2020

Characterization of human anti-hrB-like monoclonal antibody

Antoine P. Blancher, Marion E. Reid, Simone J. Alié-Daram, Jean Michel H. Dugoujon, Francis L. Roubinet

Immunohematology, Volume 12 , ISSUE 3, 119–122

Article | 03-November-2020

Use of the MAIEA assay to demonstrate that Fy3 is on the same glycoprotein as Fy6, Fya, and Fyb

The monoclonal-antibody immobilization of erythrocyte antigens (MAIEA) assay is a technique that detects trimolecular complexes formed by a human antibody and a mouse monoclonal antibody with specific red cell epitopes. This enzyme-linked immunoadsorbent assay test gives a positive reaction when two different epitopes on the same membrane protein are separately recognized by human and mouse antibodies. In this study, the MAIEA test was used to determine if the Duffy system antigen Fy3 is on the

Jaw-Lin Tzeng, Roger Dodd, Delores Mallory

Immunohematology, Volume 14 , ISSUE 3, 113–116

Article | 14-October-2020

MIMA-9, a valuable antibody for screening for rare donors

Edith Tossas, Ragnhild Øyen, Gregory R. Halverson, Harry Malyska, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 43–45

Article | 22-November-2020

New human monoclonal antibody reagents for detecting C, c, E, e, K1, Jka, and Jkb red cell antigens

Marti Kemper, Kathleen Sazama

Immunohematology, Volume 10 , ISSUE 1, 8–11

Article | 18-October-2020

Human anti-Dia monoclonal antibodies for mass screening

Toru Miyazaki, Shinichiro Sato, Toshiaki Kato, Hisami Ikeda

Immunohematology, Volume 16 , ISSUE 2, 78–81

Case report | 29-December-2020

A case report: cold hemagglutinin disease in a pancreatic and renal transplant patient

thermal amplitude of 37°C. The patie  nt had a weakly positive direct antiglobulin test (DAT) with only complement detectable on the red cells. The patient recovered spontaneously. The etiology of the CHD is unclear. The use of cyclosporin-A, OKT3 monoclonal antibody, and anti-thymocyte globulin (ATG) to treat acute rejection could have played a part.

Catherine Y. Beiting, Kathleen S. Larimore

Immunohematology, Volume 4 , ISSUE 4, 85–87

Article | 01-April-2020

Reduced red blood cell destruction by antibody fragments

period of the RBCs in the circulation. Direct injection of mouse RBC-specific Ter-119 monoclonal antibody into mice resulted in a more severe anemia compared with that in mice injected with the Ter-119 F(ab´)2 fragment. We found that mouse RBCs coated in vitro with the Ter-119 F(ab´)2 fragment, when transfused into mice,survived longer in circulation compared with RBCs coated with whole Ter-119 IgG molecule. The data support the conclusion that antibodies can be rendered less pathogenic

Amina Mqadmi, Steven Abramowitz, Xiaoying Zheng, Karina Yazdanbakhsh

Immunohematology, Volume 22 , ISSUE 1, 11–14

Article | 14-October-2020

Detection of granulocyte antibodies by flow cytometry without the use of pure granulocyte isolates

Karen M. Kiekhaefer, Karen M. Cipolone, Jo L. Procter, Kazuhiko Matsuo, David F. Stroncek

Immunohematology, Volume 17 , ISSUE 3, 70–75

Article | 09-November-2020

Semiautomation of platelet HPA-1a phenotyping by SPRCA and ELISA

samples, 13 were identified as HPA-1a negative. A further 36 known donor HPA-1a negative samples were correctly typed by both methods. Concordant results were obtained for the ELISA and SPRCA. All presumed HPA-1a negatives were confirmed by either polymerase chain reaction using sequence-specific primers (PCR-SSP) or by monoclonal antibody-specific immobilization of platelet antigens (MAIPA). The two semiautomated systems provide simple, accurate, reproducible, and secure ways for large scale HPA-1a

Lionel A. Mohabir, Lynne Porter

Immunohematology, Volume 13 , ISSUE 2, 44–48

Letter to Editor | 26-October-2020

Letters to the Editors: A macaque monoclonal antibody anti-CD44: a useful reagent for identifying the dominant type Lu(a–b–) phenotype

Antoine Blancher, Ragnild Øyen, Edith Tossas, Marion E. Reid, Frances Roubinet

Immunohematology, Volume 15 , ISSUE 2, 82–82

Case report | 14-October-2020

Neonatal alloimmune thrombocytopenia due to HPA-3a antibodies: a case report

commerical antigencapture ELISA (GTI-PakPlus kit®). Anti-HPA-3a antibodies, while weakly reactive in the monoclonal antibody immobilization of platelet antigens (MAIPA) assay in the immediate postpartum serum, were readily detectable using this assay in a sample taken 4 weeks later. Genotyping for human platelet antigens (HPA) 1–5 by the polymerase chain reaction technique with sequence-specific primers (PCR-SSP) revealed the infant’s platelet genotype to be HPA-1a/1a, 3a/3b, while that

A. Davoren, G. Smith, G. Lucas, S. Rodgers, P. O’Donoghue, J. Crowley, C.A. Barnes, J. McKiernan

Immunohematology, Volume 18 , ISSUE 2, 33–36

Report | 06-November-2019

Drug-induced immune neutropenia/ agranulocytosis

, sulfamethoxazoletrimethoprim, β-lactam antibiotics, clozapine, levamisole, and vancomycin. Assays used for detection of neutrophil drug-dependent antibodies (DDAbs) include flow cytometry, monoclonal antibody immobilization of granulocyte antigens, enzyme-linked immunosorbent assay, immunoblotting, granulocyte agglutination, and granulocytotoxicity. However, testing for neutrophil DDAbs is rarely performed owing to its complexity and lack of availability. Mechanisms proposed for DIIN have not been rigorously studied

Brian R. Curtis

Immunohematology, Volume 30 , ISSUE 2, 95–101

Article | 14-October-2020

Neonatal alloimmune thrombocytopenia due to anti-HPA-2b (anti-Koa)

; 109/L, and Hb was 116 g/L. Eleven mL of matched platelets compatible by monoclonal antibody immobilization of platelet antigens (MAIPA) assay were transfused in utero, raising the platelet count to 62 × 109/L. Repeat transfusions were done later that week and 1 week later, with pretransfusion counts of 19 × 109/L and 16 × 109/L, respectively. Delivery by C section was done at 35.5 weeks, after the third platelet transfusion. Platelet count at birth was 77 × 109/L. Drainage

Mindy Goldman, Élise Trudel, Samir Khalife, Gwendoline M. Spurll

Immunohematology, Volume 19 , ISSUE 2, 43–46

Article | 27-April-2020

Is there a relationship between anti-HPA-1a concentration and severity of neonatal alloimmune thrombocytopenia?

concentration and severity of NAIT when ELISA is used, although the correlation between ELISA and other methods, such as monoclonal antibody immobilization of platelet antigens (MAIPA) assay, remains to be determined.

Hagop Bessos, Marc L. Turner, Stanislaw J. Urbaniak

Immunohematology, Volume 21 , ISSUE 3, 102–108

Report | 16-October-2019

Mixed-field agglutination observed in column agglutination testing is not always associated with the A3 subgroup

A blood donors with either MFA or weak agglutination on routine ABO CAT were recalled. CAT was repeated with human and monoclonal anti-A, and tube agglutination testing with monoclonal anti-A and PCR-SBT of ABO exon 7 was performed. A total of 13 of the 21 donors returned, and ABO CAT with human anti-A was repeated. Eleven samples showed MFA suspected to be the A3 subgroup, and two samples showed 2+ strength suspected to be the Aweak subgroup. When tube agglutination testing using monoclonal

Nampeung Anukul, Nipapan Leetrakool, Praijit Tanan, Poonsub Palacajornsuk, Phennapha Klangsinsirikul

Immunohematology, Volume 34 , ISSUE 2, 49–56

Report | 06-November-2019

Drug-induced immune thrombocytopenia: incidence, clinical features, laboratory testing, and pathogenic mechanisms

candidate drug resulted in recurrent thrombocytopenia. Flow cytometry testing for DDAbs can be useful in confirmation of a clinical diagnosis, and monoclonal antibody enzyme-linked immunosorbent assay testing can be used to determine the platelet glycoprotein target(s), usually GPIIb/IIIa or GPIb/IX/V, but testing is not widely available. Several pathogenic mechanisms for DIIT have been proposed, including hapten, autoantibody, neoepitope, drug-specific, and quinine-type drug mechanisms. A recent

Brian R. Curtis

Immunohematology, Volume 30 , ISSUE 2, 55–65

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