Article | 04-December-2017
sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology, Volume 49 , ISSUE 3, 268–275
research-article | 30-November-2020
molecularly characterized. The sequence identities of partial 18S, ITS and 28S rDNA of Q. curvus with Q. capitatus are 95% (34 bp difference, 0 indel), 82% (99 bp difference, 44 indels) and 89% (75 bp difference, 10 indels), respectively, confirming the species separation.
The phylogenetic analyses of Q. curvus are presented in Figures 2-4. The trees inferred from 18S, and 28S analyses separated into two distinct clades (PP = 1.00, 1.00, respectively) which comprises species of subfamilies Telotylenchinae
Jianfeng Gu,
Maria Munawar,
Pablo Castillo,
Bo Cai
Journal of Nematology, Volume 53 , 1–11
Research Article | 03-September-2018
anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.
Parisa Jalalinasab,
Mohsen Nassaj Hosseini,
Ramin Heydari
Journal of Nematology, Volume 50 , ISSUE 2, 219–228
research-article | 30-November-2020
). Three sets of DNA primers (Integrated DNA Technologies, Coralville, IA, USA) were used in the PCR analyses to amplify nucleotide sequences of the partial 18S, 28S (LSU), and ITS of ribosomal RNA genes (rDNA). The partial 18S region was amplified with 1813F and 2646R primers (Holterman et al., 2006). The LSU rDNA regions were amplified using 28–81for and 28–1006rev primers (Holterman et al., 2008), and the ITS was amplified with the F194 (Ferris et al., 1993) and AB28-R primers (Curran et al., 1994
Maria Munawar,
Dmytro P. Yevtushenko,
Pablo Castillo
Journal of Nematology, Volume 53 , 1–17
Original Research | 18-July-2017
infective forms, by a broadly rounded tail end in mycetophagous females and lateral fields with 11 to 12 lines midbody in both mycetophagous females and males. The partial 18S, complete internal transcribed spacer, and partial 28S D2/D3 rRNA genes were amplified and sequenced. Phylogenetic analyses of the genes grouped the new species with previously sequenced species of Deladenus in a fully supported clade. This is the first report of Deladenus species with a known infective stage to have the excretory
Qing Yu,
Jianfeng Gu,
Weimin Ye,
Rusong Li,
Jie He
Journal of Nematology, Volume 49 , ISSUE 2, 168–176
research-article | 24-April-2020
Malorri R. Hughes,
Deborah A. Duffield,
Dana K. Howe,
Dee R. Denver
Journal of Nematology, Volume 52 , 1–6
research-article | 30-November-2019
Phougeishangbam Rolish Singh,
Beatrice E. Kashando,
Marjolein Couvreur,
Gerrit Karssen,
Wim Bert
Journal of Nematology, Volume 52 , 1–17
research-article | 30-November-2020
) determine whether nematodes putatively identified as H. thomomyos from Thomomys hosts revealed cryptic species, 2) better define the geomyid hosts parasitized by H. thomomyos, and 3) serve as the first molecular report and phylogenetic study for this species. We surveyed four Thomomys species, T. bottae, T. bulbivorus, T. talpoides (Richardson, 1828), and T. townsendii (Bachman, 1839), that occur in Oregon for intestinal nematodes. Partial 18S rRNA and COI mtDNA sequences were used to confirm the
Malorri R. Hughes,
Alexandra A. Gibson,
Emily R. Wolfe,
Cecily D. Bronson,
Deborah A. Duffield
Journal of Nematology, Volume 53 , 1–11
research-article | 30-November-2019
partial 18S and 28S D2 to D3 rRNA gene sequences, and (ii) to investigate the phylogenetic relationships of this neotylenchid nematode within the superfamily Sphaerularioidea.
Materials and methods
Sampling, extraction, mounting, and drawing
Soil, root, and wood samples were randomly collected from different regions of eastern forests in Guilan Province, Northern Iran during 2015. Nematodes were recovered directly from the wood samples by the Whitehead tray method (Whitehead and Hemming, 1965). The
Parisa Jalalinasab,
Mehrab Esmaeili,
Weimin Ye,
Ramin Heydari
Journal of Nematology, Volume 52 , 1–10
research-article | 30-November-2018
the use of morphometric measurements, morphological features, and genetic variation within the partial 18S-ITS1-5.8S-ITS2-partial 28S and 28S-D3 regions of the rDNA to characterise P. curvicauda and to distinguish it from other Pratylenchus species including those commonly found in Australia. This exercise is an important step in assessing the pest status and economic importance of the nematode to the grains industry in Western Australia.
Materials and methods
Nematode population
Soil samples
Farhana Begum,
John Fosu-Nyarko,
Shashi Sharma,
Bill Macleod,
Sarah Collins,
Michael G. K. Jones
Journal of Nematology, Volume 51 , 1–15
research-article | 30-November-2018
J. D. Eisenback,
L. A. Holland,
J. Schroeder,
S. H. Thomas,
J. M. Beacham,
S. F. Hanson,
V. S. Paes-Takahashi,
P. Vieira
journal of nematology, Volume 51 , 1–16
Research Article | 17-October-2018
of females (n = 20) included: body length 671.1 (570.1–785.3) µm; body width 32.5 (27.8–37.0) µm; onchiostyle 32.5 (31.1–34.8) µm; anterior end to esophagus-intestinal valve 117.6 (101.2–128.5) µm; a 21.5 (15.3–28.1) µm; b 5.2 (4.9–6.3) µm; V 52.9% (48.1–55.4%) µm; and vagina length 8.7 (7.8–10.7) µm. To confirm the identity of P. minor, DNA was extracted from single females (n = 3) using Extract-N-Amp™ Tissue PCR Kit (Sigma-Alredich Inc., St. Louis, MO). The partial 18S rRNA, the D2-D3 expansion
Abolfazl Hajihassani,
Negin Hamidi,
Bhabesh Dutta,
Chris Tyson
Journal of Nematology, Volume 50 , ISSUE 3, 453–455