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  • Journal Of Nematology

 

Article | 04-December-2017

Description of a New Anguinid Nematode, Nothotylenchus phoenixae n. sp. (Nematoda: Anguinidae) Associated with Palm Date Trees and Its Phylogenetic Relations within the Family Anguinidae

sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences

MEHRAB ESMAEILI, RAMIN HEYDARI, WEIMIN YE

Journal of Nematology, Volume 49 , ISSUE 3, 268–275

research-article | 30-November-2020

Morphological and Molecular Characterization of Quinisulcius curvus from China

molecularly characterized. The sequence identities of partial 18S, ITS and 28S rDNA of Q. curvus with Q. capitatus are 95% (34 bp difference, 0 indel), 82% (99 bp difference, 44 indels) and 89% (75 bp difference, 10 indels), respectively, confirming the species separation. The phylogenetic analyses of Q. curvus are presented in Figures 2-4. The trees inferred from 18S, and 28S analyses separated into two distinct clades (PP = 1.00, 1.00, respectively) which comprises species of subfamilies Telotylenchinae

Jianfeng Gu, Maria Munawar, Pablo Castillo, Bo Cai

Journal of Nematology, Volume 53 , 1–11

Research Article | 03-September-2018

Nothotylenchus andrassy n. sp. (Nematoda: Anguinidae) from Northern Iran

anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.

Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari

Journal of Nematology, Volume 50 , ISSUE 2, 219–228

research-article | 30-November-2020

Integrative taxonomy, distribution, and host associations of Geocenamus brevidens and Quinisulcius capitatus from southern Alberta, Canada

). Three sets of DNA primers (Integrated DNA Technologies, Coralville, IA, USA) were used in the PCR analyses to amplify nucleotide sequences of the partial 18S, 28S (LSU), and ITS of ribosomal RNA genes (rDNA). The partial 18S region was amplified with 1813F and 2646R primers (Holterman et al., 2006). The LSU rDNA regions were amplified using 28–81for and 28–1006rev primers (Holterman et al., 2008), and the ITS was amplified with the F194 (Ferris et al., 1993) and AB28-R primers (Curran et al., 1994

Maria Munawar, Dmytro P. Yevtushenko, Pablo Castillo

Journal of Nematology, Volume 53 , 1–17

Original Research | 18-July-2017

Deladenus posteroporus n. sp. (Nematoda: Neotylenchidae) Isolated from Packaging Wood from Canada and White Pine (Pinus monticola) Lumber from the United States and Intercepted in Ningbo, China

infective forms, by a broadly rounded tail end in mycetophagous females and lateral fields with 11 to 12 lines midbody in both mycetophagous females and males. The partial 18S, complete internal transcribed spacer, and partial 28S D2/D3 rRNA genes were amplified and sequenced. Phylogenetic analyses of the genes grouped the new species with previously sequenced species of Deladenus in a fully supported clade. This is the first report of Deladenus species with a known infective stage to have the excretory

Qing Yu, Jianfeng Gu, Weimin Ye, Rusong Li, Jie He

Journal of Nematology, Volume 49 , ISSUE 2, 168–176

research-article | 24-April-2020

First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)

Malorri R. Hughes, Deborah A. Duffield, Dana K. Howe, Dee R. Denver

Journal of Nematology, Volume 52 , 1–6

research-article | 30-November-2019

Plant-parasitic nematodes associated with sugarcane in Kilimanjaro, Tanzania

Phougeishangbam Rolish Singh, Beatrice E. Kashando, Marjolein Couvreur, Gerrit Karssen, Wim Bert

Journal of Nematology, Volume 52 , 1–17

research-article | 30-November-2020

Phylogenetics and genetic variation of Heligmosomoides thomomyos in Western pocket gophers (Thomomys spp.)

) determine whether nematodes putatively identified as H. thomomyos from Thomomys hosts revealed cryptic species, 2) better define the geomyid hosts parasitized by H. thomomyos, and 3) serve as the first molecular report and phylogenetic study for this species. We surveyed four Thomomys species, T. bottae, T. bulbivorus, T. talpoides (Richardson, 1828), and T. townsendii (Bachman, 1839), that occur in Oregon for intestinal nematodes. Partial 18S rRNA and COI mtDNA sequences were used to confirm the

Malorri R. Hughes, Alexandra A. Gibson, Emily R. Wolfe, Cecily D. Bronson, Deborah A. Duffield

Journal of Nematology, Volume 53 , 1–11

research-article | 30-November-2019

Description of Deladenus gilanica n. sp. (Hexatylina: Neotylenchidae) isolated from wood of black pine in Northern Iran

partial 18S and 28S D2 to D3 rRNA gene sequences, and (ii) to investigate the phylogenetic relationships of this neotylenchid nematode within the superfamily Sphaerularioidea. Materials and methods Sampling, extraction, mounting, and drawing Soil, root, and wood samples were randomly collected from different regions of eastern forests in Guilan Province, Northern Iran during 2015. Nematodes were recovered directly from the wood samples by the Whitehead tray method (Whitehead and Hemming, 1965). The

Parisa Jalalinasab, Mehrab Esmaeili, Weimin Ye, Ramin Heydari

Journal of Nematology, Volume 52 , 1–10

research-article | 30-November-2018

Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

the use of morphometric measurements, morphological features, and genetic variation within the partial 18S-ITS1-5.8S-ITS2-partial 28S and 28S-D3 regions of the rDNA to characterise P. curvicauda and to distinguish it from other Pratylenchus species including those commonly found in Australia. This exercise is an important step in assessing the pest status and economic importance of the nematode to the grains industry in Western Australia. Materials and methods Nematode population Soil samples

Farhana Begum, John Fosu-Nyarko, Shashi Sharma, Bill Macleod, Sarah Collins, Michael G. K. Jones

Journal of Nematology, Volume 51 , 1–15

research-article | 30-November-2018

Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

J. D. Eisenback, L. A. Holland, J. Schroeder, S. H. Thomas, J. M. Beacham, S. F. Hanson, V. S. Paes-Takahashi, P. Vieira

journal of nematology, Volume 51 , 1–16

Research Article | 17-October-2018

First Report of Stubby-Root Nematode, Paratrichodorus minor, on Onion in Georgia, U.S.A

of females (n = 20) included: body length 671.1 (570.1–785.3) µm; body width 32.5 (27.8–37.0) µm; onchiostyle 32.5 (31.1–34.8) µm; anterior end to esophagus-intestinal valve 117.6 (101.2–128.5) µm; a 21.5 (15.3–28.1) µm; b 5.2 (4.9–6.3) µm; V 52.9% (48.1–55.4%) µm; and vagina length 8.7 (7.8–10.7) µm. To confirm the identity of P. minor, DNA was extracted from single females (n = 3) using Extract-N-Amp™ Tissue PCR Kit (Sigma-Alredich Inc., St. Louis, MO). The partial 18S rRNA, the D2-D3 expansion

Abolfazl Hajihassani, Negin Hamidi, Bhabesh Dutta, Chris Tyson

Journal of Nematology, Volume 50 , ISSUE 3, 453–455

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