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Report | 01-December-2019

Effects of pH changes of stock normal saline solution on 5 percent red cell suspension

The red cell suspension (RCS) is a universally used indicator system to demonstrate antigen and antibody reactions in vitro. Saline solutions that are used in its preparation are preferred to be fresh to avoid changes in pH that may affect the results. Thus, buffered saline such as phosphate-buffered saline (PBS) is the ideal diluent because its pH is maintained for a certain period. However, normal saline solution (NSS) is more commonly used because it is inexpensive and easy to make. pH

Gregorio L. Martin, Princess Joy M. Caraan, Jared Jenrik S. Chua, Jules Albert L. Crescini, Joseph Martin C. Diokno, Christopher Bernard Dlr. Javier, Kristina Barbara O. Reyes, Rianne Y. Soliven

Immunohematology, Volume 30 , ISSUE 3, 126–134

Article | 06-December-2020

Antibody detection errors due to acidic or unbuffered saline

solid phase- or hemagglutination-based antibody detection tests. It is recommended that phosphate-buffered saline at a pH of 7.0 to 7.5 be employed.

Susan Rolih, Ron Thomas, Fern Fisher, Joanne Talbot

Immunohematology, Volume 9 , ISSUE 1, 15–18

Report | 09-October-2019

Stability guidelines for dithiothreitol-treated red blood cell reagents used for antibody detection methods in patients treated with daratumumab

if large quantities of RBCs could be treated at one time, stored, and used for testing at a later time. Panel cells were treated with DTT and then stored as three sets. Set 1 DTT-treated RBCs were stored in Alsever’s solution at 2°C to 8°C, washed daily, and suspended in pH 7.3 phosphate-buffered saline (PBS) prior to antigen typing. Set 2 DTT-treated RBCs were stored in pH 7.3 PBS. Set 3 DTT-treated RBCs were stored in Alsever’s solution. Sets 2 and 3 were inspected daily for

Wendy L. Disbro

Immunohematology, Volume 33 , ISSUE 3, 105–109

Article | 05-December-2017

Influence of Root Exudates and Soil on Attachment of Pasteuria penetrans to Meloidogyne arenaria

with root exudates of eggplant (Solanum melongena cv. Black beauty) reduced spore attachment compared with pretreatment with phosphate-buffered saline (PBS), suggesting that the nematode surface coat was altered or the spore recognition domains on the nematode surface were blocked. Spore attachment was equally reduced following exposure to root exudates from both host and nonhost plants for M. arenaria, indicating a common signal that affects spore attachment. Although phytohormones have been shown


Journal of Nematology, Volume 49 , ISSUE 3, 304–310

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