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original-report | 30-November-2020

Identification of RHD allelic variants discovered by atypical typing results on the NEO/Echo platforms

Immucor genotyping laboratory (Warren, NJ). DNA was extracted using the automated QIAcube (QIAGEN, Hilden, Germany) and was then quantified on the Nanodrop (ThermoScientific, Waltham, MA). The samples were tested by an escalating algorithm of molecular assays. First, samples were tested using RHD BeadChip (Immucor). This commercially available assay can identify 78 polymorphisms for weak and variant D alleles. If the sample had no identifiable allelic variants on the RHD BeadChip, the sample was

J.P. Hudgins, C. Matsushita, C.W. Tuma, L. O’Brien, I.A. Shulman

Immunohematology, Volume 37 , ISSUE 4, 165–170

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